THE INFLUENCE OF MAGNETOFLUIDS NANOCOMPOSITES TO POTATOES OLD VARIETIES VITROPLANTLETS
Anca BACIU*
*Research and Development Station for Potato – Targu Secuiesc, Romania
anca.mihab@gmail.com
Abstract. The genus Solanum is of great importance for the molecular and cell biology researches in general, and for the vegetal genetic engineering in particular.
Solanum tuberosum ssp. tuberosum L. is one of the most important culture plants of the temperate zone, being the fifth in rank among the cultivated species around the world and the fourth in rank as world crop and as proteins production among the culture plants.
In the scientific activity of the Department of Genetics the magneto fluids nanocomposites have been used since from 1985. From the historical point of view the magneto fluids nanocomposites were use in technology, medicine and in the plant kingdom. The most spectacular results were obtained in medicine in the carcigenesis treatment. The magneto fluids nanocomposites were used in biotechnology, especially for improving the regenerative processes.
In our experiments we wanted to understand the magneto fluids nanocomposites effect upon in vitro regeneration processes in Solanum tuberosum L. old varieties.
Keywords: potato, varieties, in vitro, magneto fluids nanocomposites
Anca BACIU*
*Research and Development Station for Potato – Targu Secuiesc, Romania
anca.mihab@gmail.com
Abstract: The author is currently involved in collecting, making an inventory, evaluation and preservation the old varieties from the Western part of Romania. In this paper 8 potato old varieties collected during 20 years and 2 varieties from National Institute of Research and Development for potato and Sugar Beet Brasov are presented. The preservation was carried out in vivo and in vitro. Important changes were observed during this time. In our work we identified many gaps in the knowledge and understanding of the origin of transformations. We made a comparison between two big areas of potato growth: Apuseni Mountains [5] and the Maramures County [3]. In these areas the potato represents the main food in winter. This work opens opportunities for future researches in the field of political and ethical decisions for potato gene pool conservation. Soon the exchange of genetic resources will be a diplomatic issue.
Keywords: potato, varieties, in vitro, molecular evaluation
Cristian-Felix BLIDAR*, Gheorghe-Emil BANDICI**, Ildikó SZABÓ***, Marian MONICA****,
Adriana PETRUŞ-VANCEA*, Liviu POP*
* University of Oradea, Faculty of Science, Department of Biology, Romania
**University of Oradea, Faculty of Environment Protection, Department of Agricultural, Romania
***University of Oradea, Faculty of Medicine and Pharmacy, Department of Botany, Romania
**** North University of Baia Mare, Faculty de Science, Department of Chemistry and Biology, Romania
cblidar@uoradea.ro
Abstract. In this study, we had proposed to dignify the Cymbidium hybridum protocorms reactions, in a vitroculture regime/condition, on liquid medium MS (1962), maintained on special filter paper bridges (Blidar type [1]), in the conditions of the presence fructose as principal sources of carbon, using various phytohormons. The basic medium culture used by us in these experiments was Murashige – Skoog (1962), without agar. To this, we added different growth regulators, such as: 2,4-D (2 mg/l), or mixture of BA (2 mg/l) with NAA (1 mg/l), or only BA (2 mg/l), or only NAA (1 mg/l). The witness lot was consisted of vitrocultivated protocorms on same liquid medium culture, without growth regulators.
At the end of the experimental period, was proved that the stronger multiplication of the protocorms number was registered using medium culture with BA cytochine in a quantity of 2 mg/l, being numerical above 1.7 times more in comparison with the control variant.
Keywords: protocorms, Cymbidium (orchid), paper-bridge, fructose, “in vitro”
DATA ON COAL DUMPS RETRIEVING IN PETROŞANI BASIN USING SEA BUCKTHORN (HIPPÖPHAE RHAMNOIDES)
Andreea BRASOVAN*, Vlad CODREA*
*Babeş-Bolyai University, Faculty of Biology and Geology, Cluj-Napoca, Romania
tbrasovan@yahoo.com
Abstract. This contribution presents data on the sea buckthorn (Hippöphae rhamnoides), with focus on its value on coal dumps retrieving in the Petroşani Basin. Due to its peculiar roots exposing a lot of nodules, this plant has an important role in fixing the atmospheric azoth and soil genesis. It is very adaptable to any kind of soil and has wide climatic exigencies, but is depending on light intensity. It has a large dissemination capacity, due to its light and numerous seeds. Besides its utilization on dump retrieving, sea buckthorn is used in medicine and pharmacy, as well as in organic fuel production.
Keywords: coal dumps, Petroşani Basin, cutting, soil fertilization.
STUDY REGARDING NITRATE AND NITRITE CONTENT IN CAULIFLOWER, FROM AGRO-FOOD MARKETS IN TIMISOARA
Bulmagă ALINA*, Lăzureanu AUREL*, Alexa ERSILIA*, Negrea MONICA*
*Banat`s University of Agricultural Science Timişoara, Romania
alinaoliveiratop@yahoo.com
Abstract. In this study we followed the monitorization of nitrate and nitrite content in caulliflower. Caulliflower samples for the analyse have been prelevate from Timisoara agro-food markets, these proceed from four location of District of Timis: Tomnatic, Jimbolia, Sânandrei şi Cenad. Nitrates (NO3), proceed from complete ammonium oxidation in the presence of nitrification enzymes, but can appear also thanks to excessive nitrogen fertilisation. The nitrites (NO2), rise from incomplete ammonium oxidation in the presence of nitrification enzymes. Those appearance indicate an advanced stadium of decomposition process of nitrogen organic substances.
Nitrate and nitrite content in cauliflower were done colorimetrically with the help of Rapid Test in the Laboratory for the Measurement of Residues of the Department of Agro-techniques of the U.S.A-V.M.B in Timisoara.
Keywords: caulliflower, nitrate, nitrite
Mariana CEVEI*, Dorina STOICANESCU**
*University of Oradea, Faculty of Medicine and Pharmacy, Oradea, Romania
**University of Medicine and Pharmacy, Department of Medical Genetics, Timisoara, Romania
cevei_mariana@yahoo.com
Abstract. Arthrogryposis is a congenital disorder characterized by multiple joint contractures found throughout the body at birth. We present the case of a 3.5 year-old girl with multiple congenital defects: arthrogryposis involving bilateral hip, knee and ankle joints, together with sacral agenesis and with lumbar dysmorphism, anorectal agenesis, hydronephrosis of the left kidney as the result from reflux, right kidney hypoplasia, renal fusion and heart anomalies: tetralogy of Fallot. Immediately after birth, in several steps, colostomy, left ureterocystoneostomy and suprapubic cystostomy were performed. Later in infancy corrective surgery for the heart defect was required. At the age of 2 years, surgery for the equinovarus deformities and for left genu flexum was performed. Medical rehabilitation tries to maximize independent function. The main goals were increasing the muscle tonus of upper-limbs, increase the rate of motion of the joints, establishment of stability for ambulation, learning different schemes of movement according to her needs, obtaining of a functional independency. The therapies were successful, after 10 weeks an improvement of the moving capacity and of the transfer in orthostatism with minimal external assistance, with the obvious increasing of the patient's satisfaction, were noticed.
Keywords: arthrogryposis, medical rehabilitation, physical therapy
CHARCOT-MARIE-TOOTH DISEASE: GENETIC AND REHABILITATION ASPECTS
Mariana CEVEI*, Dorina STOICANESCU*
*University of Oradea, Faculty of Medicine and Pharmacy, Oradea, Romania
**University of Medicine and Pharmacy "Victor Babes", Department of Medical Genetics, Timisoara, Romania
cevei_mariana@yahoo.com
Abstract. Charcot-Marie-Tooth hereditary motor and sensory neuropathy refers to a group of disorders characterized by a chronic motor and sensory polyneuropathy. Typical cases have distal muscle weakness and peroneal atrophy often associated with mild to moderate sensory loss, depressed tendon reflexes, and pes cavus. Hereditary neuropathies are categorized by mode of inheritance and chromosomal locus. The diagnosis is based on family history, characteristic findings on physical examination, EMG, nerve conduction velocity testing, and occasionally on nerve biopsy. The disorder shows allelic and non-allelic genetic heterogeneity, thus mutations of different genes leading to the same clinical features. Also, different mutations of the same gene may lead to different phenotypes. Molecular genetic testing is available in clinical laboratories for diagnosis of 7 subtypes of the disease. Genetic counseling and risk assessment depend on the inheritance. We present two cases with Charcot-Marie-Tooth type 1 and type 2 respectively. There is no cure for the disorder, although physical therapy and moderate activity are often recommended to maintain muscle strength and endurance.
Keywords: hereditary neuropathy, genetic heterogeneity, rehabilitation
STUDY REGARDING TO ASPARAGUS OFFICINALIS L. CALUS REACTION, SUBCULTURED ON ASEPTIC MEDIUM WITH VARIOUS GROWTH REGULATORS
Floare HURGOIU*, Cristian-Felix BLIDAR*
*University of Oradea, Faculty of Science, Department of Biology, Romania
hurgoiu_florica@yahoo.com
Abstract. In our studies, we investigated the effects of some growth regulators, in aseptic conditions, on obtaining Asparagus officinalis plantlets from calus, after 12 weeks of its inoculation.
The most recomanded culture media, for obtaining a high number of multimodal stems was MS basal medium, with a mixture of 1mg/l BA and 1 mg/l IBA, which inducing a considerably calusogenesis, and a lot of regenerated plantlets from it, but finally, this medium-after 12 weeks of culture initiation, induced plantlets vitrification.
Keywords: Asparagus,calus, hyperhidria, vitrification, auxine, cytokines
THE CLINIC-STATISTIC STUDY OF OSTEOPOROSIS
Florin MARCU*, Liviu LAZAR*
*University of Oradea, Faculty of Medicine and Pharmacy, Oradea, Romania
mfmihai27@yahoo.com
Abstract. Osteoporosis is the most common metabolic bone disease and is characterized by the shrinkage in bone mass and the distruction of bone quality, thus conferring a higher risk for fractures and injuries. Osteoporosis reaches clinical attention when it is severe enough to induce microfractures and the collapsing of vertebral bodies manifesting with back aches or predisposition to other bone fractures.
The aim of the study was to establish a statistic-numeric report between women and men in subjects diagnosed with osteoporosis through DEXA that present with a clinical simptomatology. We studied a group of subjects of masculine and feminine genders that have been diagnosed with osteoporosis through DEXA at the EURORAD clinic in Oradea from 01.01.2007-to present time .The result of the study was that the simptomatology of osteoporosis with pain and even cases of fractures is more obvious in female subjects then in male patients; statistically ,a woman/man report of 6.1/1 was established.
Keywords: osteoporosis, back aches, microfractures, frequency, bone mass
Oana MARE ROŞCA*, Monica MARIAN*, Lucia MIHALESCU*
*North University of Baia Mare, Faculty of Sciences, Department of Chemistry-Biology
oanarosca76@yahoo.com
Abstract. Life of zoobenthic communities that populate the watercourses is influenced by the mode of setting up of the substrate, slope, flowing speed and water flow as well as by the anthropogenic influence.
Ephemeroptera, and Plecoptera and also Trichoptera (EPT) are the main biomarkers in the evaluation of the health condition of the aquatic ecosystems. The new river classification methodologies in quality classes have been tested and mostly harmonized at European level.
This paper followed the distribution mode of EPT in the Sasar river basin and evidencing their biomarker quality.
Keywords: Normalized Global Biologic Index, EPT, monitoring biologic methods
Monica MARIAN*, Camelia VARGA*, Leonard MIHALY-COZMUTA*, Anca MIHALY COZMUTA*, Lucia MIHALESCU*, Oana ROSCA MARE*, Cristian Felix BLIDAR**
* North University of Baia Mare, Faculty de Science, Department of Chemistry and Biology, Romania
** University of Oradea, Faculty of Science, Department of Biology, Romania
moni_marian@hotmail.com; nitella_ro@yahoo.com
Abstract. This paper summarizes our research work regarding the dynamics of vegetation growth of miscellaneous species of trees planted and monitored in the particular environment of the tailing pond in Bozanta Mare (Maramures County). The structure of soil bearing high content of heavy metals and cyanides considerably impacts the ecologic conditions of tailing ponds. Aspects related to soil characteristics (such as structure, size of particles, porosity, texture, chemical composition) are included. Vegetal species that have accommodated within the tail pond are included as well. In the framework of our experiment we have planted seedlings belonging to four species of trees: Quercus petraea, Populus tremula, Betula verrucosa, Salix caprea. We have planted the seedlings in different location contexts in the tailing pond (“in situ”), as we have also planted “ex situ” witness trees. Our aim was to monitor the dynamics of growth of the stem and of cuttings. Our contribution, based on the outcomes of our research, consists in the formulation of functional correlations spotted between cormophites and micro biota, between the species of trees and their environmental underlying conditions, with the overarching goal to optimize the activities undertaken in order to alleviate the tailing ponds inherent to mining activities.
Key words: ecologic remediation, tailing ponds, dynamic of growth, main stem, cuttings, environmental underlying conditions
INFLUENCE OF THE MICROELEMENT LEAD ON THE GROWTH, DEVELOPMENT AND RESPIRATION OF ZEA MAYS PLANTLETS
Lucia MIHALESCU*, Monica MARIAN*, Oana MARE ROŞCA*
*North University of Baia Mare, Faculty de Science, Department of Chemistry and Biology, Romania
luciamihalescu@yahoo.com
Abstract. The purpose of this paper is to observe the effect of the microelement lead on some physiological processes of the corn plantlets (Zea mays). The parameters linked to the germination, to the variation of the growth of the radicular system and the aboveground parts, as well as the variation of the respiration.
Following this study we can state that high quantities of lead produce the reducing of the germinating speed due to the inhibition of cell division.
High concentrations of lead cause the death of plants towards the last measuring days block the enzymes, affect the plasmatic membrane permeability.
The highest value of the respiration intensity is recorded for the plants grown in high concentration solutions (Pb 0.1% and Pb 0.01%).
Keywords: plantlets, lead, growth, variant, respiration
Luiza MIKE*, Anca BACIU*, Daniela POPA*, Zsuzsanna NEMES*
*Research and Development Station for Potato – Targu Secuiesc, Romania
scdc@clicknet.ro
Abstract. Potato, from the breeding point of view, presents the following biological physiological specific features: vegetative multiplication, heterogeneity of progenitors’, large plasticity, sterility, incompatible to hybridization, excessive sensibility to diseases. The first three specific features advantage the breeding process on a large scale, but the last two thus complicates the breeding activity and constitutes important problems to success.
The breeding of potato has as permanent objective the obtaining new varieties with high yield capacity, with high resistance to diseases and pest, with high quality, which have to give the satisfaction to consumers.
The creation of new potato varieties is a continue process, which has to take into account the change of ecological conditions, with increase of aggressively and pathogenity of diseases and pest, apparition of rases, stems, brotypes, pathotipes, as well as the continue increasing of consumers demands.
Keywords: potato, new varieties, yield capacity, resistance to viruses, chips and pommes frites
Luiza MIKE*, Anca BACIU*, Daniela POPA*, Ion BOZESAN**, Dumitru BODEA***
* Research and Development Station for Potato – Targu Secuiesc, Romania
** National Institute of Research and Development for potato and Sugar Beet Brasov, Romania
*** Research and Development Station for Agriculture Suceava, Romania
scdc@clicknet.ro
Abstract. A large number of potato varieties and distinguished breeding lines disappeared as an effect of nonfavourable climatically conditions and especially by viruses diseases, as well as other biological and viruses degeneration. To avoid the negative effect of degeneration on potato varieties and distinguished breeding lines, the method of selection for maintaining and multiplication of potato is applying in Romania in the frame of National Center for Maintaining of potato varieties and distinguished breeding lines Apa Rosie, Covasna County, which belong to the Station for Research and Development of Potato, Targu Secuiesc, Covasna County.
In this center are maintained and multiplied all distinguished varieties and breeding centers from Romania (National Institute for research and Development of Potato and Sugar beet Brasov, Research and Development Station for Agriculture Suceava, Research and Development Station for Potato Targu Secuiesc, Research and development Station for Potato Miercurea Ciuc).
Using the method of selection for maintaining it is possible an early identification of somatic mutations, disease (especially viruses) infection by visual elimination or by serological testing.
The viruses’ infection of potato leads to disturbed the metabolism of plants and produces anatomical – morphological alters as: mosaic, crinkle, rolling, browning of leaves and plants deformation.
The disturbing of plant metabolism has as negative effect the reduction of vegetation period, decreasing the yield capacity, depreciation of physical and chemical quality of tubers.
The genetically complex structure of cultivated potato (2n = 4x = 48) and strong segregation of long – expected characters in the obtained future progeny by sexual hybridization, complicated many times by nonfavourable linkage, are the backgrounds for initiation of maintain selection.
Keywords: potato, selection of maintaining, biological purity
RESEARCHES CONCERNING NITRATES AND NITRITES ACCUMULATION IN CARROTS, ALONG OF THE VEGETATION STAGES
Negrea MONICA*, Lăzureanu AUREL*, Alexa ERSILIA*, Bulmagă ALINA*
*Banat`s University of Agricultural Science Timişoara, Romania
negrea_monica2000@yahoo.com
Abstract. The presented paper deals with the determination of nitrates and nitrites content in carrots, in different vegetation stages of the carrot culture. High nitrates and nitrites concentration in vegetables is mainly due to excessive nitrogen content in the soil system, thus deteriorating the nutritional and hygienic values of products and complicating the processing and storage. The determination was tested on carrot samples assayed from an experimental field set up near Timisoara. In experimental field, to the carrot culture was administrated different doses of fertilizers (NPK) and the samples for analysis were assayed in different phases of vegetation. The obtained results indicated that the highest level of nitrate in carrots was found to the variant b3 (N150P90K90) in experimental field, who was above maximum limit allowed (LMA). Maximum limit allowed for nitrates in carrots, in accordance with ORDER No. 293/640/2001-1/2002 regarding security and quality conditions for vegetables and fresh fruits for human consumption is 400 ppm. For all other samples of carrots the nitrates level was below of LMA. The nitrite content grows in case of fertilizer administration during the whole vegetation stages of the plant. In variant N150P90K90 the nitrite content was above (LMA) in carrot samples in all stages of vegetation. The nitrite content in carrots should not exceed 1-2 ppm. Nitrate and nitrite content in carrots was done with the help of High Performance Liquid Chromatography (HPLC) in the Laboratory for the Measurement of Residues of the Department of Agro-techniques of the U.S.A-V.M.B in Timisoara.
Keywords: carrots, nitrate content, nitrite content, HPLC
THE STUDY OF THE POTATO’S LIFE-CYCLE PHASES IMPORTANT TO THE INCREASE OF THE INDIVIDUAL VARIABILITY
Zsuzsanna NEMES*, Anca BACIU*, Daniela POPA*, Luiza MIKE*,
Adriana PETRUS – VANCEA**, Oana DANCI***
* Potato Research and Development Station, Târgu-Secuiesc, Romania
**University of Oradea, Faculty of Science, Department of Biology, Romania
***Banat University of Agricultural Sciences and Veterinary Medicine Timisoara, Department Genetic Engineering in Agriculture
zsuzsa20@yahoo.com
Abstract. The authors describe the practical importance of the knowledge of the potato’s life-cycle phases. There was made a research about the life-cycle of some varieties created at the Potato Research and Development Station Targu-Secuiesc, aiming at the proper management of the crop care, the irrigation and fertilization of the crops, depending on their objective.
The varieties Redsec, Ioana, Luiza and Star are semi-late maturing varieties, with a vegetative period of 80 – 110 days, each of them displaying certain specific features during the growth and development period that are highlighted in the present research paper.
Keywords: potato, life-cycle, variability, production
Zsuzsanna NEMES*, Daniela POPA*, Anca BACIU*, Luiza MIKE*
*Potato Research and Development Station – Targu Secuiesc, Romania
zsuzsa20@yahoo.com
Abstract. In the period 2002-2003 under laboratory conditions it was determined the influence of biological parameters (temperature, relative air humidity, energetically and plastically resources and the composition of the cultivation environment) on the growth of the Phytophthora infestans fungus colonies.
The biological parameters determined in vitro for the Ph. infestans fungus showed that the fungus needs mesophyll conditions. The energetically and plastically resources are important to its growth, being indispensable to the colonies’ evolution.
Keywords: Ph. infestans, biological parameters, development, colonies
Ioan OSWALD*, Simona BIRSAN**
*University of Oradea, Faculty of Pharmacy and Medicine, Romania
**Medical clinically recovery hospital Baile Felix, Romania
Abstract. Our study have a collaboration interdisciplinary with looking at product magnetic field and your effect in medical pathology. I built one current generative which give a modulation current in form tooth of saw with frequency between 30-40Hz. Now instruments with which work therapy with magnetic field generation currents with sinusoidal modulation for example BIOMAG or MTU 500 with invariable frequency 50Hz and 100Hz or rectangular modulation currents with variable frequency between 0-50Hz, at instrument type BTL used very much.
The coils with generate magnetic field product our instruments are using instrument type BTL. Induction electromagnetic at two coils, ( cervical and lumbar), is highest 12mT and at local coils highest 23Mt.
The results in therapeutic pathology I make through to compare with obtains classic instruments. In follow our study we have obtain:
- to all patients in our study I using only continuo form
- through compare with other instruments we obtain good results, in specially rheumatic and inflammations disease
- we have type of patients which not response these therapy
- in case articulations legs we have to decrease a number sessions from 21 to 14 for a induction 12mT to apply 20 minutes
Keywords: magnetic fields, theraphy, coil, sinusoidal modulation
MITOCHONDRIAL DISFUNCTION AND APOPTOSIS IN LEUKEMIA CELLS
Annamaria PALLAG*, Marcela BOTA**, Aurel ARDELEAN***, Ladislau RITLI**,
Ildiko SZABO*, Mariana MURESAN **
* Specialization Pharmacy, Faculty of Medicine and Pharmacy, University of Oradea, Romania
** Specialization Medicine, Faculty of Medicine and Pharmacy, University of Oradea, Romania
***”Vasile Goldiş” Western University of Arad, Romania
inachisio@rdslink.ro
Abstract. Apoptosis or programmed cell death is a process which involves the intentional degradation of the cell from the inside, the participation of the mitochondria to propagate the apoptotic signal, the alteration of the phospholipid cell membrane composition, the perturbation and alteration of the cell metabolism.
The antineoplastic drugs is inducing the apoptotic process in the sensitive cells.
It have been studied acute lymphoblastic leukemia cells. Using Annexin V-PE Apoptosis Detection Kit and flow cytometer, the amount of cells undergoing apoptosis, in various stages of the antineoplasic treatment, was detected. At the same time, were monitored, the serum level of malondialdehyde. The results obtained confirm the alteration of the mitochondrial metabolism. We can observed the mitochondrial dysfunction role in cell apoptosis.
Keywords: mitochondria, apoptosis, leukemia, cell metabolism, reactive oxigen species
Cristian Mircea PETRUŞ*, Dorina CACHIŢĂ – COSMA**
* University of Oradea, Faculty of Science, Department of Biology, Romania
**”Vasile Goldiş” Western University of Arad, Department of Biology, Romania
adrianavan@yahoo.com
Abstract. We proposed in this experiment to study the spraying effect – during 30 days –on the foliar limbs of Tradescantia sp. cuttings leafs, and on the rooting substratum, made with Pi water (PiW) or deuterium depleted water (DDW) (with 25 ppm D), concerning rhisogenesis and plantlets growth index, or about stomata apparatus, identified at the level of inferior epidermis of foliar limb. In the end we concluded that Pi water proved to have a stimulator effect on Tradescantiacuttings organogenesis, by foliar or radicular application. The deuterium depleted water increased only the rhisogenesis, by foliar spraying, comparatively to the control lot (foliar limb and basal cuttings sprinkled with distilled water - DW), which stomas were numerous at the level of foliar limbs of the plantlets sprinkled, to the base, with Pi water and foliar with distillate water.
Keywords: Pi water, deuterium depleted water, Tradescantia, organogenesis, stomata
EPIDERMAL FORMATIONS OF CYMBIDIUM INVITRO- AND EXVITROPLANTLETS
Adriana PETRUŞ - VANCEA*, Gheorghe Emil BANDICI**, Anca BACIU***,
Cristian - Felix BLIDAR*, Liviu POP*
* University of Oradea, Faculty of Science, Department of Biology, Romania
**University of Oradea, Faculty of Environment Protection, Department of Agricultural, Romania
***Potato Research & Development Station from Târgul –Secuiesc, Romania
adrianavan@yahoo.com
Abstract. Adaptation to septic medium is dependent to gas change between exvitroplantlets and outside medium, this is why we decided to study the Cymbidium and modifications which appear in vitroculture at stoma density, dimension and closing system, and also the capacity of this vitroplantlets to adaptation its antideshydratation defence system, during acclimatization period, using as standard measure those plants which were cultivated to septic medium. After results analyze we observed that the Cymbidium hybridum species have amphystomatical leaf only “in vitro” medium conditions, and to greenhouse plants and exvitroplantlets the leafs are hypostomatic. This situation is generated from his special nutrition which exists in vitrocultures. This fact was explained by Salisbury(1927) [8] and Schoch (1978) [9], who demonstrated that the stoma frequency may significant vary to some as ambient factors which modify the morphology and composition of leaf.
Keywords: stoma, vitroplantlets, exvitroplantlets, acclimatization, Cymbidium
THE CORRELATIONS BETWEEN PHENOTYPICAL CHARACTHERS IN SELECTED FAMILIES BY GENTIANA LUTEA L.
AND THE CHEMICAL COMPOUNDS OF UNDERGROUND ORGANS
Mihai Radu POP*, Camelia SAND*, Horea BARBU*
*“Lucian Blaga” University of Sibiu, Department of Biology, Romania
mihaipop76@yahoo.com
Abstract. The ground organs of Gentiana lutea L. contain active principles, which have certain properties recommended for anorexia or convalescence.
Along the year we tried to show cast the existent correlations between the main morphological characters and the productivity elements, such as the concentration in active principles. It is proved that the development of a character influence either the development of positive correlation or negative correlation [7].
Having in mind that the phonotypical expression is influenced by the phases of vegetation, a very important thing to know is the morph chemical characteristics of specie in different phases of vegetation.
The superior quality of the raw materials obtained by different sowing methods defined during our research, will decrease the crop from our country wild flora [10].
Keywords: Gentiana lutea L., active principles, correlations, selected families
Mihai Radu POP*
*“Lucian Blaga” University of Sibiu, Department of Biology, Romania
mihaipop76@yahoo.com
Abstract: Angelica archangelica L. species represents a floristic rarity, is declared endangered species, its harvesting from spontaneous flora not beeing permitted [4]. For obtaining vegetal material for industrialization, this is realized by cultivation of the species [8].
Modern ameliorating has the tendence to enrich scientific , using methods that will permit the acceleration of process and accuracy by diminuation of his probabilistic character. Using of molecular markers is inscribed in the same tendency, wich consists in one of the most efficient methodes wich offers an important opportunity in analizing, administrating and using of genetic variability through RAPD technique.
Keywords: Angelica archangelica L.,RAPD, dendrogram
Daniela POPA*
*Potato Research and Development Station, Targu-Secuiesc, Romania
daniela_ralpopa@yahoo.com
Abstract. The research was carried on at Targu - Secuiesc within 2002-2004 and its objective was to determine the most efficient fungicides to control the late potato blight caused by the fungus Phytophthora infestans. All the applied products were efficient in controlling the disease in comparison to the untreated control variant.
The results of the research show that during the observed period the products Curzate and Acrobat proved to be highly efficient in a constant manner at the three varieties: Ostara, Sante and Desiree. The lowest frequency of infected tubers was registered at the varieties treated with Altima, Acrobat, Tatoo C.
Keywords: Phytophthora infestans, fungistats, control, potato
Daniela POPA*
*Potato Research and Development Station – Târgu Secuiesc, Romania
daniela_ralpopa@yahoo.com
Abstract. This paper presents the behaviour of some potato varieties and lines towards the attack of the Phytophthora infestans (Mont) de Bary fungus under the field conditions of the Potato Research and Development Station, Targu-Secuiesc. During the observed period (2001-2003) the appearance of new forms of attack was registered, for example the attack on the stem, on the leaf petioles, as well as a greater number of infected tubers. The greatest number of blighted stems belonged to the varieties Sante and Ostara, and the smallest one was registered in the case of the variety Lady Roseta, followed by Desiree and Nemere. The results prove that there is a direct relationship between the frequency of the attack on the stems and that of the blighted tubers in the case of every variety, except for the varieties Lady Roseta and Nemere at which the smallest number of infected tubers was registered.
Keywords: Phytophthora infestans, varieties, attack on the stems, the frequency of blighted tubers
THE INITIATION OF A TROPIC SHRUB SPECIA
PSIDIUM GUAJAVA
Julieta-Emilia ROMOCEA*, Cristian-Felix BLIDAR*, Liviu POP*
*University of Oradea, Faculty of Science, Department of Biology, Romania
Abstract. Because this tropical fruit is not so popular in Europe, we sis try he initiation of an tropic shrub of Psidium guajava it was possible to make, using them seeds from the matured fruit of guava. The fruit is originally from Egypt – Alexandria. Those seeds were dry and before using them, they were kept in sterile water few hours, after that it was performed the sterilization process, and they were inoculated in 4 different experimental variants.
Because them germination process was start late, after 2 months from inoculation, observations were made to the level of the germinated seeds, didn’t shown any infections, but the best results were noticed only on variant V1 (BM basic medium - MS with BA (1 mg/l) + IBA (1 mg/l)), where the germination capacity it was more bigger.
Finally, we did noticed that after the end of this experiment, the best medium culture for the generation of stemlets with many leaves is V1 and V3, but for the root development only V2 showed a very good result. Kept in good light intensity, humidity and optimal temperature conditions, the experiment showed good results, what made this research possible.
Keywords: Psidium guajava, vitrocultures, growth regulators, fruits, C vitamin
HISTO-ANATOMY OF THE STEM OFSOLANUM TUBEROSUM L.
Monica ŞIPOŞ*, Manuela ILEA*, Iosif MOLNAR*, Daniel BUNTA*
* University of Oradea, Faculty of Science, Department of Biology, Romania
siposmonica@yahoo.com
Abstract. The paper reveals the anatomical structure of the stem of Solanum tuberosum L. in the incipient stages of the ontogenetic development (the popular denomination „sprout”). Cross sections of the stem of this species were performed. The appearance of the secondary structure – even in this early ontogenetic stage – was confirmed by the presence of the cambium and the tissues generated by this. As a result, at the potato, the purely primary structure of the stem is of a short duration.
Keywords: Solanum tuberosum L., stem, anatomy
HISTO-ANATOMICAL AND HISTOGENETIC ASPECTS IN THE STEM AND ROOT OF CHELIDONIUM MAJUS L.
Monica ŞIPOŞ*, Iosif MOLNAR*, Manuela ILEA*, Dorina GURĂU**
* University of Oradea, Faculty of Science, Department of Biology, Romania
**Greek-catholic School, Oradea, Romania
siposmonica@yahoo.com
Abstract. The present study focuses on aspects of anatomy and histogenesis in the root and stem of common celandine (Chelidonium majus L.). The stem is annual, having a primary structure. It is to be noticed the depositing parenchyma within the medullar rays and around the vascular bundles. The secretory tissue from the near vicinity of the primary phloem gets together with this in the composition of the collateral vascular bundles. The chlorenchyma initially present in the structure of the common celandine stem shall be subsequently replaced by depositing and mechanic tissues. The primary perennial root has a diarch secondary structure. This structure is due to the functioning of the cambium and the phellogen along several years. The secondary phloem is a deposit for the amyloplasts, there being present here several laticifers. The ritidoma is well represented, black in colour, also rich in laticifers. The secondary roots have a medullar origin and disticha distribution.
Keywords: Chelidonium majus L., stem, root, anatomy
Eliane-Teodora STĂNCIOIU*, Ioan GODEANU*
*Unversity of Craiova, Fac. de Horticultură
elianne_s@yahoo.com
Abstract. In Romania, the establishment of nut tree plantations is continuously expanding, yet the seeding material production has never raised up to the level of cultivators requirements.
In our country, although the mother plants are confirmed as Targu Jiu 1, Secular and recently Portval, because of lack of seed tree materials, at present, most of the varieties are being grafted on saplings proceeded from a mixture of genotypes belonging to the species of Juglans regia.
By studying the rich stock of germoplasma, present in the district of Gorj, 20 hybrids, that have appropriate features to their formation as mother plants have been collected, so that they might improve the existing variety.
Keywords: Juglans regia, Juglans nigra, nut tree, graftid
IMPLICATION OF GENETIC FACTORS IN OSTEOPOROSIS SUSCEPTIBILITY
Dorina STOICANESCU*, Mariana CEVEI**
* “Victor Babes” University of Medicine and Pharmacy, Timisoara, Romania,
**University of Oradea, Medicine and Pharmacy Faculty, Felix Spa Clinical Rehabilitation Clinique, Romania
dstoicanescu@gmail.com
Abstract. Osteoporosis is a multifactorial disease characterized by a decrease in bone mass and deterioration of bone architecture. Genetic factors are determinants of peak bone mass and may influence age-related decreases of bone mass. WHO has established an operational criterion based on bone density measuring, the T-score. 122 cases were studied, 51 diagnosed with primary osteoporosis. Mean age was 57.6 years. The main inclusion criterion was the acceptance of affected individuals to participate in the study. Accurate family history was taken. Daughters of affected persons were evaluated by DEXA technique. 67.2% of them had T-score values that indicated osteopenia or osteoporosis (mean value -2.3SD). In conclusion, descendents of affected parents are at a high risk for osteoporosis, important aspect for primary prevention.
Keywords: osteoporosis, T-score, family history
PREVALENCE OF MINOR CONGENITAL ANOMALIES IN CHILDREN WITH HYPERACTIVITY
Dorina STOICANESCU*, Mariana CEVEI**
*University of Medicine and Pharmacy "Victor Babes", Department of Medical Genetics, Timisoara, Romania
**University of Oradea, Faculty of Medicine and Pharmacy, Oradea, Romania
dstoicanescu@gmail.com
Abstract. A congenital anomaly is a physical, metabolic, or anatomic deviation from the normal pattern of development that is present at birth. Minor congenital anomalies do not have medical or cosmetic importance, but detection of more than three such anomalies may reveal the prenatal origin of a disorder. The aim of the present study was to establish an association between the presence of minor congenital anomalies and hyperactivity, learning disabilities and attention deficit. 219 patients aged between 7 and 18 years were examined for the presence of minor congenital anomalies and compared to a control group. The frequencies of these anomalies were not significantly different in the two groups. The mean values of minor congenital anomalies per child were significantly different: 2.5 in children with learning disabilities, 4 in those with learning disabilities and attention deficit and hyperactivity, 1.05 in the control group. Prevention is the best approach to congenital anomalies. As a positive correlation could be established, it was concluded that the presence of minor congenital anomalies especially more than three, may predict the future onset of these disorders.
Keywords: minor congenital anomalies, hyperactivity, predictive value
