ARTICLES 2009 #2                        
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VANADIUM IN MEDICINAL PLANTS: NEW DATA ON THE OCCURENCE OF AN ELLEMENT BOTH ESSENTIAL AND TOXIC TO PLANT AND MAN

Diana Simona ANTAL*, Cristina Adriana DEHELEAN*, Carmen Maximiliana CANCIU*, Manfred ANKE**

* University of Medicine and Pharmacy "Victor Babes", Department of Pharmacy, Timisoara, Romania
**Friedrich-Schiller-Universität Jena, Institut für Ernährungswissenschaften, Dornburgerstr. 24, D-07743 Jena, Germania
Corresponding author: Diana Simona Antal, Department of Pharmacy, "Victor Babes” University of Medicine and Pharmacy,2 Eftimie Murgu Square, 300041 Timişoara, Romania, tel. /fax: 0040256220479, e-mail: diana.antal@umft.ro 

Abstract. The biological importance of the trace element vanadium on a triple level – nutritional, pharmacological and toxicological – determined us to perform a large-scale investigation of medicinal plants in what their vanadium content is concerned, and to evaluate the extraction ratio of this element onto herbal teas. The analysis of 56 species, carried out through Inductively Coupled Plasma - Mass Spectrometry (ICP-MS), revealed an average V content of 502 μg/kg dry matter in medicinal plants. The highest V content was found in flowering aerial parts, with an average of 763 μg/kg, followed by leaves (682 μg/kg), roots (600 μg /kg ), flowers (352 μg/kg) and fruits (112 μg/kg). Wild thyme (Thymus pulegioides) has a particular capacity to accumulate this element; other V-rich species are Geum urbanum, Urtica dioica, Hypericum perforatum and Valeriana officinalis. Given the antidiabetic effect of V, wild thyme may be interesting supplement in diabetes mellitus type II. The extraction ratio of Rb through decoction ranges between 13% - 85%, according to the investigated species and plant organ; V from subterranean parts is best extractable. The present research presents the first large-scale analysis of Romanian plants with regard to their vanadium content.

Keywords: vanadium, medicinal plants, ICP-MS, aqueous extraction.

 

 

RESULTS REGARDING NEW ROMANIAN POTATO (Solanum tuberosum L.) CULTIVARS REACTION TO IN VITRO CULTURE CONDITIONS

Anca BACIU*, Adriana PETRUŞ – VANCEA**, Zsuzsanna NEMES*, Robert MOTICA*, Luiza MIKE*

* Potato Research and Development Station, Targu-Secuiesc, Romania
**University of Oradea, Faculty of Science, Department of Biology, Oradea, Romania
Corresponding author: Anca Baciu, Potato Research and Development Station, 55 Ady Endre Str., 525400 Targu-Secuiesc, Romania,
tel.: 0040267363755, fax: 0040267361770, e-mail: anca.mihab@gmail.com

Abstract. The Solanum genus presents a great importance for research due to its economical importance being a great aim to different breeding programs. It is the best represented genus from the Solanaceae family, part of this family species producing tubers. Working method used in our experimentations was double node fragments culture. Our studies aimed regeneration and multiplication of four potato cultivars (Desirée, Redsec, Ts. 95-1161-66 and Ts. 94-1117-98) in order to improve the multiplication and pathogen free material obtaining protocols. Biometrical determinations performed for explants and new plantlets made possible to conclude as the best in vitro response was given by the cultivar Redsec both regarding the regeneration, growth and multiplication capacityand in foliar surface evolution, overcoming even the control (Desiree variety) and the cultivar Redsec can be recommended for other in vitro experimentations being a potent cultivar for this type of culture.

Keywords: potato varieties, in vitro cultures, regeneration and micropropagation

 

 

THE INFLUENCE OF SAMPLES INCUBATION ON DETECTION OF PLRV AND THE EFFECT OF SOME EXTRACTION BUFFER’S ADDITIVES ON THE DETECTION OF POTATO VIRUSES Y, A, X AND S BY ELISA TECHNIQUE

Carmen Liliana BADARAU*, Nicolae COJOCARU*, Sorin Nicolae RUSU*, Maria IANOŞI*, Krisztina PETRUSCA*

*National Institute of Research and Development for Potato and Sugar Beet Braşov, Romania
Corresponding author: Carmen Liliana Badarau, National Institute of Research and Development for Potato and Sugar Beet Braşov, 2 FundĂturii, 500470 Brasov, Romania, tel. 0040268476795, fax: 0040268476608, e-mail: carmen_badarau@yahoo.com

Abstract. The goal of this research was to examine and evaluate the effects of several modifications of ELISA (enzyme linked immunosorbent assay) technique on the detection of potato viruses Y, A, X, S and potato leafroll virus by this technique. These modifications consisted on: modification of the incubation modality of conjugate (IgG-AP), the use of several additives in extraction buffer, replacement of grinding buffer with McIlvain buffer.
The results show a better identification of PLRV in leaves and sprouting tubers using the co-incubation sample and IgG-AP conjugate. Compared with the classical method, the test safety and sensitivity increased. Testing leaves, the average values of OD at 405 nm was 5-6,6 times higher than those obtained by standard DAS ELISA method and using sap from sprouting tubers (dilution 1/10) this average was 1,5 times higher.
The detection of potato viruses Y and A by enzyme–linked immunosorbent assay (ELISA) can be improved using extraction buffers with new composition. Using McIlvain’s phosphate-citric acid buffer (0,18M; pH 7), the absorbance values (A405nm) increased significantly for PVY and PVA detection comparing with the classic extraction buffer. Sodium diethyldithiocarbamate (0,01M) in phosphate-buffered saline plus Tween 20 (PBS-T) used instead of the polyvinylpyrrolidone increased the sensitivity of potato virus Y but this additives decrease the absorbance values in case of PLRV identification. The same decrease was observed when we used sodium thioglicolat (0,01M) and sodium diethyldithiocarbamate (0,01M) in PBS-T. The co-incubation sample and conjugate and the use of McIlvain’s buffer could save time and costs of potato viruses diagnostic tests.

Keywords: potato virus, co-incubation, extraction buffer, ELISA.

 

 

CORRELATIONS BETWEEN SEVERAL GENE POLYMORPHISMS
AND BREAST CANCER

Alina BELENGEANU*, Andrei ANGHEL**, Elena LAZAR***, Dorina STOICANESCU****

* University of Medicine and Pharmacy "Victor Babes", Department of Cell and Molecular Biology, Timisoara, Romania
**University of Medicine and Pharmacy "Victor Babes", Department of Biochemistry, Timisoara, Romania
***University of Medicine and Pharmacy "Victor Babes", Department of Anatomopathology, Timisoara, Romania
****University of Medicine and Pharmacy "Victor Babes", Department of Medical Genetics, Timisoara, Romania
Corresponding author: Alina Belengeanu, Department of Cell and Molecular Biology, University of Medicine and Pharmacy “Victor Babes”, 2 E. Murgu Square, 300041 Timisoara, Romania, tel.: 0040256204476, e-mail: alinabele@yahoo.com

Abstract. Breast cancer is among the most common forms of cancer. The molecular mechanisms involved in hormone dependence of breast cancer have been largely investigated. The role of the estrogen and progesterone receptors has been documented, but the role of androgen receptor is less well known. A great part of the researches is focused on identifying the gene profile for disease predisposition, as well as the gene profiles characteristic to different stages of the disorder. Gene instability is the result of a minor alteration at the level of the microsatellites from the genomic DNA sequence. Changes of the stability of microsatellites are considered markers in the colon cancer and in different types of solid tumors. Breast cancer samples were analyzed to determine the existence of several polymorphisms in the structure of the genes that code for hormone receptors such as estrogen, progesterone and androgen receptors. The following tandem repeats were sized by gel electrophoresis: CA for ER-α, TA for ER-β and CAG for androgen receptor, together with PROGINS polymorphism. Correlated with the stage of malignancy it was noticed that majority of cases with a higher number of CAG repeats as well as those who had the allele of 481 bp in PROGINS segment were in stage III or IV, with invasive ductal carcinoma and a severe prognosis. It was also noticed that polymorphisms of estrogen receptors genes may be correlated with a severe prognosis, but due to the low number of samples, no ferm conclusions could be drawn.

Key Words: gene polymorphism, estrogen receptor, androgen receptor, PROGINS fragment, breast cancer

 

 

THE REACTIVITY OF Cymbidium hybridum PROTOCORM-LIKE BODY VITROCULTIVATED IN LIQUID MEDIUM, DEPLETED IN MICROELEMENTS

Cristian-Felix BLIDAR*, Gheorghe-Emil BANDICI**, Ildikó SZABÓ***,
Felicia CODOBAN-ȘCHIOP****, Nicoleta GROZA-GANEA****

* University of Oradea, Faculty of Science, Department of Biology, Oradea, Romania
**University of Oradea, Faculty of Environment Protection, Department of Agricultural, Oradea, Romania
***University of Oradea, Faculty of Medicine and Pharmacy, Department of Pharmacy, Oradea, Romania
**** Master’s Student at University of Oradea, Faculty of Science, Department of Biology, Oradea, Romania
Corresponding author: Cristian-Felix Blidar, University of Oradea, Faculty of Science, Department of Biology, 1 Universitatii Str., 410087 Oradea, Romania, tel.: 0040259408161, fax: 0040259408461, e-mail: cblidar@uoradea.ro

Abstract: The nature of the chemical compounds, the proportion and concentration in mineral elements, as part of the culture medium’s preparation, they influence the growth and the morphogenesis of clones. Starting from these premise, we proposed ourselves to study the Cymbidium hybridum protocorms reactivity, in the cultivation conditions of those in submersing regime in liquid medium Murashige-Skoog (1962) (MS) [14], with a complete set of mineral elements, or damaged in one of those microelements as B, Mn, Zn, Mo, Cu, Co or I. Those culture medium contained or not various growth hormones.
After 90 days from inoculation, the most proliferative intense processes of the protocorms, we have registered in the practiced vitrocultures without iodine microelement, but in the presence of BA (2 mg/l) in the liquid medium, as a unique growth regulator (reference lot, considered 100%), the registered increases were 600% in the protocorms multiplication. The absence of iodine in the culture medium has determined the increase of the fresh and dry weight of the protocorms’ biomass, regardless the growth hormones content in the substratum, except the medium with 2 mg/l 2,4-D, from which, the performed observations made after 30 days from the experiment’s montage, were noticed senescence processes.

Keywords: protocorm-like body (PLB), Cymbidium (orchid), microelements, growth regulators, “in vitro”

 

 

ENZYMOLOGICAL STUDY OF THE EVOLUTION OF THE TECHNOGENIC SOIL SUBMITTED TO BIOLOGICAL RECULTIVATION IN BOZANTA MARE (MARAMURES COUNTY, North-Werstern Romania)

 Cristian Felix BLIDAR*, Liviu POP*, Adriana PETRUȘ-VANCEA*

* University of Oradea, Faculty of Science, Department of Biology, Oradea, Romania
Corresponding author: Cristian-Felix Blidar, University of Oradea, Faculty of Science, Department of Biology, 1 Universitatii Str., 410087 Oradea, Romania, tel.: 0040259408161, fax: 0040259408461, e-mail: cblidar@uoradea.ro

Abstract. This paper summarizes our research work regarding the dynamics of vegetation growth of miscellaneous species of trees planted and monitored in the particular environment of the tailing pond in Bozanta Mare (Maramures County). The structure of soil bearing high content of heavy metals and cyanides considerably impacts the ecologic conditions of tailing ponds. Aspects related to soil characteristics (such as structure, size of particles, porosity, texture, chemical composition) are included. In the framework of our experiment we have planted seedlings belonging to four species of trees: Quercus petraea, Populus tremula, Betula verrucosa, Salix caprea. Our aim was to study the evolution of enzyme activities. Our contribution, based on the outcomes of our research, consists in the formulation of functional correlations spotted between cormophites and enzyme activities, between the species of trees and their environmental underlying conditions, with the overarching goal to optimize the activities undertaken in order to alleviate the tailing ponds inherent to mining activities. 

Keywords:  catalase, dehydrogenase, techonogenic soils, recultivation,

 

GLANDULAR TRICHOMES AND ESSENTIAL OIL COMPOSITION OF
Thymus pannonicus All. (Lamiaceae)

Irina BOZ*, Ioan BURZO**, Maria-Magdalena ZAMFIRACHE*, Constantin TOMA*, Claudia PADURARIU*

* „Al.I.Cuza” University, Faculty of Biology, Iași, Romania
** University of Agronomic Sciences and Veterinary Medicine, Faculty of Horticulture, Bucureşti, Romania
Corresponding author: Irina Boz, „Al.I.Cuza” University of Iași, Faculty of Biology, Carol I Bd., 20A, 700506 Iasi, Romania, tel: 0040749036689, e-mail: irina.boz@bioresearh.ro

Abstract: Glandular trichomes of Lamiaceae are among the most investigated secretory structures. Micromorphological and anatomical analyses of the glandular trichomes of Th. pannonicus L. were carried out using SEM (Scanning Electron Microscopy). Our research results show that, the secretory structures are always multicellular, consisting in a basal cell, a unicellular pedicel and a gland which bears 1, 2, 8 or 12 cells. Aerial parts of Th. pannonicus L. were subjected to hydrodistillation to yield volatile oil which were subsequently analysed by GC/MS (gas chromatography coupled with mass spectrometry). The main compounds identified in Th. pannonicus L. volatil oil were α-terpinyl acetate (48.83%), germacrene D (12.12%), cariophyllene oxide (6.35%) and mircene (4.73%).

Keywords: Thymus pannonicus L., glandular trichomes, micromorphology, volatile oil.

 

 

THE CONTENT DETERMINATION OF CALCIUM, MAGNESIUM AND ASCORBIC ACID IN SEA BUCKTHORN FRUITS AT VULCAN COAL DUMP

Andreea BRASOVAN*, Vlad CODREA*, Vasilica MÂNDROC**, Ramona CÂMPEAN***, Neli OLAH****

* “Babeş-Bolyai” University, Faculty of Biology and Geology, Cluj-Napoca, Romania
** Liviu Rebreanu” Highschool, Turda, Romania
*** Babeş-Bolyai” University, Faculty of Environmental Science, Cluj-Napoca, Romania
**** S.C. PLANTEXTRAKT S.R.L., Cluj-Napoca, Romania
Corresponding author: Andreea Brasovan, “Babeş-Bolyai” University, Faculty of Biology and Geology, 1 KogĂlniceanu, 400084 Cluj-Napoca, Romania, tel.: 0040722598075, e-mail: tbrasovan@yahoo.com

Abstract: The study presents data on content determination of calcium, magnesium and ascorbic acid in sea buckthorn fruits collected from Vulcan coal dump. The dump was never covered with soil or fertilizers containing azoth, potassium and phosphorus. The sea buckthorn was planted directly on the sterile and it has an important function in stabilizing the acclivity and fixing the atmospheric azoth with the nodosities on the roots. So, the sea buckthorn becomes widespread plant in coal dumps because it helps to the soil formation. The results obtained after the determination of calcium, magnesium and ascorbic acid from the fruits of this plant were compared with the values already reported in various references, concluding that these differences are insignificant (3.7% for magnesium and 9.4% for calcium). Therefore, the sea buckthorn from the dump can be used in food industry, as well as in pharmaceutics and medicine.

Keywords: Romania, Petroşani Basin, coal dumps, Sea buckthorn, content of calcium, magnesium, ascorbic acid.

 

 

ANTIOXIDATIVE ACTIVITY OF ETHANOL EXTRACTS FROM Spirulina platensis AND Nostoc linckia MEASURED BY VARIOUS METHODS

Liliana CEPOI*, Ludmila RUDI*, Vera MISCU*, Angela COJOCARI*, Tatiana CHIRIAC*, Daniela SADOVNIC*

* Institute of Microbiology and Biotechnology of Academy of Sciences of Moldova, ChișinĂu, Moldova
Corresponding author: Liliana Cepoi, Institute of Microbiology and Biotehnology, 1, Academiei, 2028 MD, ChișinĂu, Moldova, tel.: 0037322735524, fax: 0037322725754, e-mail: lilianacepoi@yahoo.com

Abstract. The goal of this work is to determine the level of antioxidative activity of various ethanol extracts from Spirulina platensis and Nostoc linckia biomass, and also to demonstrate the possibility to select suitable methods for evaluation of these characteristics. The methods for determination of antioxidative activity were selected concerning their possible use for complex preparations: phosphomolybdenum method for evaluation of antioxidant capacity (PMRC), radical-scavenging activity by DPPH method (DPPH), antioxidant activity by the ABTS+ radical cation assay (ABTS), Folin-Ciocalteu reducing capacity (FCRC). We showed the presence of antioxidative substances in ethanol extractions from 2 species of cyanobacteria, and possibility to increase their activity varying ethanol concentration. It facilitates the extraction both water- and lipid-soluble components from biomass. Regarding used methods for antioxidative activity determination, we have used only those based on reaction of electrons return (which widely used nowadays in vitro). Obtained in different ways results demonstrate high reduction capacity of the extracts and possibility to select suitable analytical methods for each case.

Keywords: Spirulina platensis, Nostoc linckia, antioxidative activity, ethanol extracts

 

 

IMPLICATION OF GENETIC FACTORS IN NEURAL TUBE DEFECTS ETIOLOGY

Mariana CEVEI*, Dorina STOICANESCU**

*University of Oradea, Faculty of Medicine and Pharmacy, Department of Medicine, Oradea, Romania
**University of Medicine and Pharmacy "Victor Babes", Department of Medical Genetics, Timisoara, Romania
Corresponding author: Mariana Cevei, MedicalClinicalRehabilitationHospital Felix Spa, 417500 Felix, Romania, tel. 0040723281865, e-mail: cevei_mariana@yahoo.com

Abstract. Neural tube defects are a group of developmental abnormalities that results from failure of fusion of the caudal neural tube, representing one of the most common group of malformations of human structure. The etiology is thought to be multifactorial in the vast majority of the cases, but other possible causes are heterogeneous and include chromosome abnormalities, single gene disorders and teratogenic exposures. However, the exact cause is not known in most cases and etiology remains rather complex and poorly understood. The aim of this study was to provide evidence for a genetic contribution to the etiology of neural tube defects. Multifactorial defects have a significant genetic component that interacts with specific environmental factors. Our study aimed to identify some of these risk factors, but it also established an association between the occurence of neural tube defects with spontaneous abortions, aswell as with other congenital defects, that may be considered markers of early prenatal damage. Concordance of these defects in families also sustained implication of genetic factors in their etiology.

Keywords: neural tube defects, spina bifida, genetic factors

 

 

CONTRIBUTIONS TO THE KNOWLEDGE DIVERSITY OF LIGNICOLOUS MACROMYCETES (BASIDIOMYCETES) FROM CĂPĂŢÂNII MOUNTAINS

Ioana CIORTAN*

*,,Alexandru. Buia” Botanical Garden, Craiova, Romania
Corresponding author: Ioana Ciortan,,,Alexandru Buia” Botanical Garden, 26 Constantin Lecca Str., zip code: 200217,Craiova, Romania, tel.: 0040251413820, e-mail: ciortanioana@yahoo.com

Abstract. This paper presents partial results of research conducted between 2005 and 2009 in different forests (beech forests, mixed forests of beech with spruce, pure spruce) in CĂpĂţânii Mountains (Romania). 123 species of wood inhabiting Basidiomycetes are reported from the CĂpĂţânii Mountains, both saprotrophs and parasites, as identified by various species of trees.

Keywords: diversity, macromycetes, Basidiomycetes, ecology, substrate, saprotroph, parasite, lignicolous

 

 

THE ACIDOPHILIC MICROORGANISMS DIVERSITY PRESENT IN LIGNITE AND PIT COAL FROM PAROSENI, HALÂNGA, TURCENI MINES

Carmen Madalina CISMASIU*

*RomanianAcademy, Institute of Biology, Center of Microbiology, Bucharest, Romania
Corresponding author: Carmen Madalina Cismasiu, Romanian Academy, Institute of Biology, Center of Microbiology,  296 Spl. Independenţei, 56-53 P.O. Box, 060031 Bucharest, Romania, tel.: 004021239072,  fax:  0040212219071, e-mail: carmen.cismasiu@ibiol.ro; madalinabio@yahoo.com

Abstract. Pollution from coal combustion is the largest problem in the current use of coal and the biggest constraint on the increased use of coal. When these fossil fuels are combusted, sulphur-di-oxide is released into the atmosphere causing acid rains which dissolves buildings, kills forest.
Knowing the physiological groups of microorganisms present in the coal samples has an ecological importance, completing the knowledge in the field of the microorganism’s ecology and a practical importance, being a source of new microorganisms with biotechnological potential.
The microbial communities evidenced in such sites include both groups of chemolithotrophic microorganisms involved in the metals biosolubilization processes and groups of heterotrophic microorganisms involved in the processes of bioaccumulation or biofixation of metallic ions.
In this context, this paper presents the study regarding the main physiological groups of microorganisms present in the pit coal and lignite samples after the industrial processing of coal. The results revealed that the microorganisms belonging to the following physiological groups: aerobic heterotrophic acidophilic bacteria, strictly anaerobic heterotrophic (sulphur-reducing), nitrifying bacteria (nitrite and nitrate bacteria), denitrifying bacteria and acidophilic chemolithotrophic bacteria on Fe2+, on S0 and on S2O3.

Keywords: acidophilic bacteria, coal, biodesulphurization.

 

 

EXPERIMENTAL BIOFILMS WITHIN DRINKING WATER TREATMENT PLANT ORIGIN; EVALUATION OF NUTRIENT CONCENTRATION AND TEMPERATURE INFLUENCES UPON THEIR DEVELOPMENT

Anca FARKAS*, Brînduşa BOCOŞ**, Ştefan ŢIGAN***, Corina MUREŞAN*, Romeo CHIRA*

* Someş Water Company Cluj-Napoca, Romania
** ”Prof. dr. Iuliu Moldovan” Public Healh Institute of Cluj-Napoca, Romania
*** ”Iuliu Haţieganu” University of Medicine and Pharmacy, Cluj-Napoca, Romania
Corresponding author: Anca Farkas, Someş Water Company Cluj-Napoca, 79 21December 1989 Boulevard, 400604 Cluj-Napoca, Romania, tel./fax 0040264371818, e-mail: farkasanca@yahoo.com

Abstract. From the planktonic free-floating state, microorganisms pass to the solid state, the biofilm, cells being strongly attached to each other and usually to the interface. This changing in cells’ behavior induces surface colonization and complex interactions development within the biofilm. If the biofilm’s role into the natural aquatic habitats is, undoubtedly, a positive one, consisting in water self-purification, drinking water pipe networks biofouling can be responsible for a wide range of water quality and operational problems.
This exploratory experiment was performed in order to investigate, in a time interval of 7 days, the influence of certain environmental factors such as nutrient concentration and temperature upon in vitro biofilm’s development, origin in the biofilm of water treatment plant. The method used for in vitro biofilm growth monitoring is the colorimetric measurement of the biomass.
Descriptive analyses, including the mean value, variability, trends, correlations and graphic displays were performed. The correlation analysis shown that the biofilm development in the discussed experiment was influenced as by the origin source as by the temperature, time and nutrients concentration. The biomass increment was significantly different for the biofilms with clarifier and sand filter sites origin, grown at 22˚C, while at 8˚C, the differences were not significant from a statistical point of view.
For all the dilutions, moments and temperatures considered, the biofilm’s development with clarifier origin registered was significantly higher than the biofilm with sand filter origin.

Keywords: biofilm, drinking water treatment, biofouling, colorimetric measurement of biofilm density

 

 

TESTING THE SENSITIVITY OF Staphylococcus aureus ANTIBIOTICS

Marioara Nicoleta FILIMON*, Aurica Breica BOROZAN**, Smaranda Laura GOŢIA***,
Roxana POPESCU****, Vasile Daniel GHERMAN*****

*WesternUniversity of Timișoara, Faculty of Chemistry-Biology-Geography, Department of Biology, Timişoara, Romania
**BanatUniversity of Agricultural Sciences and Veterinary Medicine from Timisoara, Departament of Horticulture, Timişoara, Romania
***University of Medicine and Pharmacy “Victor Babeş” Timișoara, Department of Physiology, Timişoara, Romania
****University of Medicine and Pharmacy “Victor Babeş” Timişoara, Cellular and Molecular Biology, Timişoara, Romania
*****University of Polytehnic, Timisoara, Faculty of Hydrotechnic, Department of Microbiology, Timişoara, Romania
Corresponding author: Marioara Nicoleta Filimon, WesternUniversity of Timișoara, Faculty of Chemistry-Biology-Geography, Department of Biology, 16 Pestalozzi, 300115 Timişoara, Romania, Tel.: 0040256592646, fax:0040256592622, e-mail: nicoleta_filimon@yahoo.com

Abstract. This study has in view to establish and test the sensitivity of Staphylococcus aureus antibiotics. There are different injuries caused by superficial skin infections: from simple pimples to infections that endanger our lives, like an abscess, furuncle septicemia, meningitis, toxic food, urinary tract infection at sexually active young women. Samples have been taken from 30 people with staphylococcus infections. They were nineteen women and eleven men, between the age of 2 and 79. During this study some antibiograms have been made, based on pharyngeal exudates, acne secretion and urine culture. It has been established that the most efficient recommended antibiotics are: oxacilin, erythromycin, rifampicin and ciprofloxacin. The penicillin turned out to be less efficient to remove and destroy the Staphylococcus aureus species.

Keywords: antibiogram, antibiotics, Staphylococcus aureus

 

 

ANATOMICAL AND MICROMORPHOLOGICALPARTICULARITIES OF VEGETATIVE ORGANS IN ENDEMIC Erysimum wittmanii Zaw. ssp. wittmanii

Irina Neta Gostin*

*”Al. I. Cuza” University, Faculty of Biology, Iasi, Romania
Corresponding author: Irina Neta Gostin,”Al. I. Cuza” University, Faculty of Biology, 11 Carol I Boulevard, 700506 Iasi, Romania, tel.: 0040232201510, fax: 0040232201472, e-mail: irinagostin@yahoo.com

Abstract: The Erysimum wittmanii ssp. wittmanii is an endemic biennial herbaceous species in South and East Carpathians. In this study the anatomical and micromorphological properties of this specie are investigated. Anatomical properties of root, stem and leaves were underlined. Roots acquire early in ontogenesis the secondary structure. Stems presents collateral vascular bundles both in primary and in secondary structure; cortical small bundles by concentric type, was observed on the cross sections from the top of the stem. The leaves are bifacial and amphystomatic. Scanning electron microscopy investigation regarding upper and lower epidermises of leaves in different developmental stages was carried out. Trichomes occur on both faces of young leaves. The tector hairs are multicelullar, usually three-armed; rarely four-armed or two-armed (especially on the epidermis from the veins). The hairs cell walls are thin and the surface is smooth in the first developmental stages (on very young leaves); on the young and mature leaves the trichomes have thick-walled cells, densely covered by micro-papillae.

Keywords: endemic, stem, root, leaves, trichomes, SEM.

 

 

SCANNING ELECTRON MICROSCOPY INVESTIGATIONS REGARDING
Adonis vernalis L. FLOWER MORPHOLOGY

Irina Neta Gostin*

*”Al. I. Cuza” University, Faculty of Biology, Iasi, Romania
Corresponding author: Irina Neta Gostin,”Al. I. Cuza” University, Faculty of Biology, 11 Carol I Boulevard, 700506, Iasi, Romania, tel.: 0040232201510, fax: 0040232201472, e-mail: irinagostin@yahoo.com

Abstract: The floral morphology of Adonis vernalis L. was observed with a scanning electron microscope (SEM). The investigations are important to clarify some taxonomical problems and also could provide useful diagnostic elements for the identification of this medicinal plant in powdered materials. All floral organs are initiated spirally and centripetally and develop centripetally. The petals (8-12) are shorter than the sepals (5-6) in early developmental stages. The petals are disposed on spiral (with 3-4 whorls). The stamens (numerous) are unbranched and reach maturity centripetally; they are free of the perianth. The anther walls consisting of a single layer epidermis in the anther wall surrounding the sporagenous tissue, one row of endothecium, two to four rows of middle layer and one row of tapetum layer. In the anther walls, the tapetal cells, by glandular type, persist later in ontogenesis. Pollen grains are tricolpate with echinate surface. The gynoecium is multiple, apocarpous with distinct carpels. The carpels are ascidiate from the beginning. At the base of each carpel, numerousness short, unicellular, trichomes are present. The stigma differentiates as two crests along the ventral slit of the ovary. Each carpel contains a single ovule inside the ovary cavity. The mature ovule is anatropous, with two integuments. It is almost parallel to the funicle.

Key words: anther,flower, pollen, ovary, ovule, tapetum.

 

 

MORPHO-ANATOMICAL CHANGES OF PLANT VEGETATIVE ORGANS OF OLIVE (Oleea europaea) TREE CULTURE TREATED WITH PESTICIDES

Anastasia MILIOU*, Adriana PETRUS-VANCEA**, Adrian Vasile TIMAR***,
Ioana Maria BORZA***, Anca BRATA***

* Archipelagos, Institute of Marine & Environmental Research of Aegean Sea, Samos, Greece.
**University of Oradea, Biology Department, Faculty of Science, Oradea, Romania.
***University of Oradea, Faculty of Environmental Protection, Oradea, Romania.
Corresponding author: Anastasia Miliou, Archipelagos, Institute of Marine & Environmental Research of Aegean Sea, Research base of Marathokampos, P.O. Box. 229, Ormos, Marathokampos, 83102 Samos, Greece, tel.: 00306974744949, e-mail: anastasia@archipelago.gr

Abstract. The objectives of this study were to assess and identify structural changes caused by pesticide treatment in Olea europaea olive tree cultures and Avena fatua plants from olive groves. For this purpose morpho-anatomical characteristics were assessed on shoots, roots and leaves to correlate the effect of pesticide treatment with anatomical and morphological aspects of leaves. This study concluded that the leaves treated with pesticides have less stomata and more numerous tector hairs as an adaptation to toxic treatment, compared with organic leaves from untreated cultures. These leaf abnormalities can seriously affect the efficiency of respiration, photosynthesis and the hydric control of the plants. Large intercellular spaces were observed in the foliar mesophyll of Olea europaea treated with pesticides. Differences in vegetative aerial organs were observed between treated and un-treated Avena fatua samples, namely the central parenchyma of stem was not reabsorbed and there were more layers of hypodermic sclerenchyma in treated leaves.
The results of the study should be heeded as a warning for all olive producers who use pesticides excessively and in an uncontrolled manner, in addition to encouraging the wider implementation of organic farming methods.

Keywords: Olea europaea, Avena, morpho-anatomy, pesticides.

 

 

FERTILIZATION OPTIMIZATION IN THE CASE OF THE PRODUCTIV POTATO VARIETY THROUGH THE REFINEMENT OF THE ROMANIAN CHEMICAL FERTILIZERS IN THE TARGU SECUIESC DEPRESSION

Zsuzsanna NEMES*, Anca BACIU*, Robert MOTICA*, Luiza MIKE*

*Potato Research and Development Station, Targu-Secuiesc, Romania
Corresponding author: Zsuzsanna Nemes, Potato Research and Development Station, 55 Ady Endre Str., 525400 Targu-Secuiesc, Romania, tel.: 0040267363755, fax: 0040267361770, e-mail: scdc@clicknet.ro

Abstract. Profitable potato cultures cannot be developed without chemical and/or organic fertilization. The fertilization must assure the best possible valorization of the intensive varieties’ production potential under the ecological conditions existing in the cultivation region.
The experiments took place at the Potato Research and Cultivation Station Targu Secuiesc in the period 2005-2007.
Our research proposed this approach to the aspects of potato fertilization in the case of the Productiv variety, created at the Potato Research and Cultivation Station Targu Secuiesc.
To achieve a higher production level, 7 levels of fertilization and 3 types of fertilizers were studied, representing N:P:K rates of 1:0:0 (ammonium nitrate), 1:1:0 (Complex 20:20:0), respectively 1:1:1 (Complex 15:15:15).
The highest and most stable productions are achieved by adding nitrogen in doses of 150-200 kg active substance / ha, and the presence of phosphorus and potassium up to these levels provides a maximum production at the application of the binary fertilizer, Complex 20:20:0, as well as at the application of the fertilizer Complex 15:15:15.

Keywords: potato, variety, fertilization, nitrogen doses

 

 

OTHER NATURAL HABITATS TYPES
(UNDER HABITAT DIRECTIVE 92/43/EEC) IN ROMANIA

Adrian OPREA*, Ion SÂRBU*

*Botanic Garden, „Alexandru Ioan Cuza” University, Iasi, Romania
Corresponding author: Adrian Oprea, Botanic Garden, „Alexandru Ioan Cuza” University, 7-9 Dumbrava Rosie, 700487 Iasi, Romania, tel.: 0040232201373; fax: 0040232201385; e-mail: aoprea@uaic.ro

Abstract. New habitat natural types are proposed in this paper. Some of the already existing natural habitats are too largely described in the "Interpretation Manual of European Union Habitats - EUR 27". That why, as a result of a scientific project developed in Romania between the years of 2001 and 2004, namely inventoring the most important plant areas to be preserved in Romania we identified other natural habitats, whose distributions cover pretty large areas in our country. As a result we proposed other 17 new habitats or habitat subtypes to be taken into considerations in the future versions of the "Interpretation Manual of European Habitats".

Keywords: natural habitats, biodiversity, Romania

 

 

POSSIBLE IMPLICATION OF LECAM-1 GENE P213S POLYMORPHISM IN THE
RISK FOR ADVANCED STAGES OF DIABETIC NEPHROPATHY IN PATIENTS WITH TYPE 1 DIABETES

Nicolae Mircea PANDURU*, Danut CIMPONERIU**, Pompilia APOSTOL**, Maria MOTA***, Eugen MOTA***, Monica STAVARACHI**, Mihai TOMA**, Irina RADU**, Anne-Marie CRACIUN*,
Cristian SERAFINCEANU*, Dan Mircea CHETA*

* ”N. C. Paulescu” National Institute for Diabetes Nutrition and Metabolic Diseases, Bucharest, Romania
**Human Genetics and Molecular Diagnostic Laboratory, Institute of Genetics, University of Bucharest, Romania
*** Diabetes Nutrition and Metabolic Diseases Department, Craiova University of Medicine and Pharmacy, Romania
Corresponding author: Nicolae Mircea Panduru, “N. C. Paulescu” National Institute for Diabetes Nutrition and Metabolic Diseases, 2nd Diabetes Clinic, 5-7 Ion Movila, 020475 Bucharest, Romania, tel. 0040314199693, fax. 0040314199918, e-mail: nicoly_pmn@yahoo.com

Abstract. Diabetic nephropathy has an unclarified pathogenesis, with multifactorial aetiology which includes metabolic and haemodynamic abnormalities, aberrant signaling of numerous cytokines or growth factors and genetic susceptibility. Inflammation and low birth weight seems to be predisposing factors for diabetic nephropathy. In both processes levels of L-selectin (CD62L) may play an important role. The genetic heritability of diabetic nephropathy and CD62L levels sustain the investigation of relationship between LECAM polymorphisms and the disease risk. The aim of the study was to investigate a possible relationship between Pro213Ser polymorphism in LECAM-1 gene and advanced stages of diabetic nephropathy in type 1 diabetes. We enrolled unrelated Caucasian patients with type 1 diabetes mellitus, fall into control group – diabetic patients with duration of disease over 20 years without microalbuminuria (n = 83) and nephropathy group – patients with overt nephropathy or end stage renal disease – ESRD (n = 121). Pro213Ser polymorphism genotyping was achieved using PCR-RFLP technique. Genotype spread analysis indicates that ProSer and SerSer are more frequent in the nephropathy group (ProSer = 41.95% and SerSer = 6.02 %), compared with the control group (ProSer = 20.53% and SerSer = 1.73%). The corrected OR's due to the small number of patients pointed the possibility that the 213Ser is the risk allele (ORSer = 1.634; p=0.04), and 213Pro is the protective one (ORPro = 0.602; p=0.04) regarding diabetic nephropathy. Thus our results suggest a possible association between the P213S polymorphism and advanced stages of diabetic nephropathy in type 1 diabetic patients. Additional researches are required in order to acknowledge the mentioned results and to clarify the mechanism by which this polymorphism intervenes in the disease pathogenesis.

Keywords: Pro213Ser polymorphism, diabetic nephropathy, type 1 diabetes, LECAM-1 gene.

 

 

THE CCl4 ACTION UPON PHYSIOLOGICAL INDICES IN
 Lepus timidus AND THE PROTECTIVE ROLE OF SOME SUBSTANCES

Alina PĂUNESCU*, Cristina Maria PONEPAL*, Octavian DRĂGHICI*, Alexandru Gabriel MARINESCU*

*University of Pitesti, Faculty of Science, Department of Ecology, Pitesti, Romania
Corresponding author: Alina PĂunescu, University of Pitesti, Faculty of Science, Department of Ecology, Targu din Vale Str., no.2, 410087 Piteşti, Romania, tel.: 0040248219709, fax: 0040248219709, e-mail: alina_paunescu@yahoo.com

Abstract. Aim of this study is to demonstrate the hepatoprotective role of grape seed oil and Cynara scolymus leaf extract. This experiment lasted for 12 days performed on male and female rabbit. The animals were intoxicated in the latest day of experiment (day 12th) with CCl4 in a dose of 30µl/100g body weight and a group of them was treated for 12 days with 0.4 mg/kg body weight/day of Cynara scolymus (artichoke) leaf extract while another group was treated with grape seed oil (1ml/kg body weight/day). Intoxication with CCl4 caused an increase a glycemia and a number of leukocytes, decrease cholesterol, triglycerides value, and a number of erythrocytes. We observed that the extract of Cynara scolymus leaf and the grape seed oil had a protective effect against CCl4 intoxication.

Keywords: Cynara scolymus, grape seed oil, glycemia, triglycerides, cholesterol, erythrocytes and leukocytes

 

 

SOME STUDENTS LEARNING STYLE PARTICULARITIES

Adriana PETRUŞ-VANCEA*

* University of Oradea, Faculty of Science, Department of Biology, Oradea, Romania
Corresponding author: Adriana Petruş-Vancea, University of Oradea, Faculty of Science, Department of Biology, 1 Universitatii Str., 410087 Oradea, Romania, tel.: 0040259408161, fax: 0040259408461, e-mail: adrianavan@yahoo.com

Abstract. The investigation aimed two objectives, namely to study the relationship between the learning styles, strategies, motivation, orientations and opinions with regard to learning, of Biology Domain students, comparative with those from double domain Biology – Chemistry, and particular learning styles of students from different specialties, which are in the first year of study, comparative with those which are in the last year of cycle I (age III of study), under the Bologna system. A version adapted by Trif, in 2007 [1], of the Learning Style Inventory (ILS), designed by Vermunt and Rijswijk (1998), was administrated to the total number of 77 students. Students of Biology specialization (Bologna system) were largely learning style oriented to understanding and at those of the Biology-Chemistry (last generation of the old system) we identified a style based on reproduction, but the differences of learning strategies and motivations, orientations and opinions were not statistically significant between the two groups of students. The second hypothesis formulated by us proved to be true, identifying significant statistically differences between the strategies, motives and opinions about learning of first academic year students, who prefer step by step learning or external guidance, learning orientation being to note, to obtain a degree, wishing much more support from teachers or colleagues (expressing an undirected learning style), compared with third academic year students, which use concrete processing of information, with getting a job motivation.

Keywords: learning style, learning strategies, students, double specializations, age of study.

 

 

ROOTS AND LEAFS HYSTO-ANATOMY OF Cymbidium hybridum CULTIVATED
„IN VITRO” AND „EX VITRO”

           
Adriana PETRUŞ-VANCEA*, Monica ŞIPOŞ*

* University of Oradea, Faculty of Science, Department of Biology, Oradea, Romania
Corresponding author: Adriana Petruș-Vancea, University of Oradea, Faculty of Science, Department of Biology, 1 Universitatii Str., 410087 Oradea, Romania, tel.: 0040259408161, fax: 0040259408461, e-mail: adrianavan@yahoo.com

Abstract. In this experiment we tried to found structural differences of Cymbidium hybridum roots and leaflets depending on culture conditions (“in vivo”, “in vitro” or “ex vitro”). At the end of the experiment we concluded that there are no structural differences between plants, except the normal ontogenetic changes. So, at the level of “in vivo” roots were observed many conductive fascicle, comparatively with “in vitro” or “ex vitro” roots, and at the “in vivo” and the “ex vitro” roots structure was identified the methaxylem, while, at the “in vivo” roots, this elements does not appear. The vitro- and exvitroleaflets mark a low chloroplast density, comparatively with control lots (“in vivo” cultivated leafs).

Keywords: Cymbidium, hysto-anatomy, vitroculture, exvitroculture

 

 

RELATIONSHIP BETWEEN HEPATITIS B AND C VIRUS PREVALENCE
AND RISK FACTORS

Roxana POPESCU*, Doina VERDES*, Laura GOTIA**, Emila NICOARA***, Nicoleta FILIMON****,
Gabriela MUTIU*****, Florin BOGDAN*****

*University of Medicine and Pharmacy “Victor Babes”, Department of Cellular and Molecular Biology, Timisoara, Romania
**University of Medicine and Pharmacy “Victor Babes”, Physiology, Timisoara, Romania
***University of Medicine and Pharmacy “Victor Babes”, Infectious Disease, Timisoara, Romania
****WesternUniversity of Timisoara, Faculty of Chemistry – Biology – Geography, Department of Biology, Timisoara, Romania,
*****University of Oradea, Faculty of Medicine and Pharmacy, Department of Microbiology, Oradea, Romania
Corresponding author: Roxana Popescu, Department of Cellular and Molecular Biology, University of Medicine and Pharmacy “Victor Babes”, 2 Eftimie Murgu, 300041 Timisoara, Romania, Tel.: 0040723649886, e-mail: popescurxn@yahoo.com

Abstract: Hepatitis C virus (HCV) and hepatitis B virus (HBV) are major causes for liver disease worldwide and potential causes for substantial morbidity and mortality in the future. The aim of this study was to assess the prevalence of hepatitis C and B virus infection and to identify the associated risk factors of viral hepatitis. In our experiments we use as materials 107 patients, 48 men and 59 women, aged from 7 to 67 years old. The maximum incidence of HBV and HCV hepatitis was observed at patients aged between 40 to 60 and especially at people in urban areas. The number of cases with HCV was twice higher at men than at women, while for HVB the incidence was higher for men than for women. Risk factors for transmission of HCV and HBV were medical procedure in the high-risk group, while vertical or sexual transmission belongs to the low-risk patient group. In conclusion, this study demonstrates that the prevalence of HBV and HCV infection is high, thus asepsis and antisepsis measures should be strictly followed.

Keywords: hepatitis C virus, hepatitis B virus, prevalence, risk factors

 

 

STUDIES REGARDING THE INFLUENCE OF ACETYLSALICILIC ACID ON SOME PHYSIOLOGICAL PROCESSES IN SUNFLOWER (HELIANTHUS SP.) SEEDLING

Cornelia PURCĂREA*, Dorina CACHIŢĂ-COSMA**

*University of Oradea, Faculty of Environmental Protection, Department of Agricultural, Oradea, Romania
** ”Vasile Goldis” WesternUniversity of Arad, Department of Biology, Arad, Romania
Corresponding author: Cornelia PurcĂrea, University of Oradea, Faculty of Environment Protection, Department of Agricultural, 26 Magheru Str., 410048 Oradea, Romania, tel.: 0040259412550, fax: 0040259416274, e-mail:neli_oradea@yahoo.com

Abstract. In this paper we studied the influence of exogenous acetylsalicylic acid (ASA) solutions administrated in different concentrations (0.01 mM, 0.1 mM, 0.5 mM, 1.0 mM and 5.0 mM) to sunflower seeds – by presoaking it for 6 hour before germination -on some physiological and biochemical processes including: plant growth and development of plants, total absorption capacity of roots and assimilatory pigments content of the first leaves of  sunflower seedlings, in comparison with the same parameters of the control lots which were treated with tape water. The results showed that exogenous 0.1mM ASA solutions, administrated to the sunflower seeds significantly increased the length of the sunflower seedlings, and the total absorption capacity of roots. The 5.0 mM ASA solution determined a very significant inhibition of the physiological processes. Spraying the primary leaves of sunflower with 0.1 mM ASA solution significantly increased the total chlorophyllian and carotenoid pigments content, and more concentrated solutions significantly decreased this parameter.  

Keywords: sunflower, acetylsalicylic acid, root, growth, total absorption capacity, primary leaves, chlorophyllian pigments,carotenoid pigments.

 

 

ANATOMY OF THE VEGETATIVE ORGANS AT
Syngonium podophyllum Schott.

Monica ŞIPOŞ*, Anamaria TRIF*

* University of Oradea, Faculty of Science, Department of Biology, Oradea, Romania
Corresponding author: Monica Şipoş, University of Oradea, Faculty of Science, Department of Biology, 1 Universitatii Str., 410087 Oradea, Romania, tel.: 0040259408161, fax: 0040259408461, e-mail: siposmonica@yahoo.com

Abstract. This article has as an objective establishing the structure of the vegetative organs at Syngonium podophyllum. The structure is specific for herbaceous monocotyledonous: root has a primary structure, the stem primary structure is an intermediary form between an aerial stem and a rhizome (the presence in a fundamental parenchyma of the colaterally closed vascular bundles and the leptocentric ones). The leaf petiole has the suberified epidermis. It is characterised by the colaterally closed vascular bundles disposed peripherically and extremely well protected by the sclerenchyma and in the centre of the petiole, in the fundamental parenchyma, the same type of fascicles are placed. The middrib has a structure similar to that of the petiole. The leaf mesophyll is homogenous. The parenchyma of aerial vegetative organs of this species is crossed by aeripherous channels and their cells contain calcium oxalate crystals. The leaf is amphistomatic, the stomatic complexes are an amarilidaceous type, tetracitic or hexacitic.

Keywords:Syngonium podophyllum , anatomy, vegetative organs

 

 

PHOSPHATIDYLINOSITOL 3-KINASE P85 REGULATORY SUBUNIT GENE AND SPINAL MUSCULAR ATROPHY DISEASE

Monica STAVARACHI*, Mihai TOMA*, Pompilia APOSTOL*, Danut CIMPONERIU*, Irina RADU*,
Nicolae Mircea PANDURU**, Niculina BUTOIANU***, Lucian GAVRILA*

*University of Bucharest, Institute of Genetics, Romania
** ”N.C.Paulescu” National Institute for Diabetes Nutrition and Metabolic Diseases, Bucharest, Romania
*** “Al. Obregia” Clinical PsychiatryHospital, Bucharest, Romania
Corresponding author: Monica STAVARACHI, University of Bucharest, Institute of Genetics, Aleea Portocalelor, No.1-3, Sector 6, 060101, Bucharest, Romania, tel: 0040213181576, fax: 0040213181565, e-mail: monica.stavarachi@gmail.com

Abstract. Spinal muscular atrophy (SMA) is a frequent neuromuscular disorder caused by motoneuronal apoptosis, as a result of SMN (Survival Motor Neuron) protein deficiency. Although the SMA determining gene was identified, the molecular mechanism of the disease is not clearly understood, due to the heterogeneity of clinical manifestations. Trying to complete the molecular describing SMA picture, by identifying potential modulators factors, we investigated the relationship between phosphatidylinositol 3-kinase p85 regulatory subunit gene (PIK3R1) and SMA pathology. As IGF signaling pathway has been reported to play an important role in motoneurons survival and PIK3 is a key element of this cascade signaling, we focused on the relationship between PIK3R1 gene Met326Ile polymorphism and SMA type I, the most severe form of the disease. A total of 80 subjects (40 SMA type I patients and 40 unrelated healthy controls) were included in the study. The statistical analyzes performed consequently to the genotyping by mismatch PCR-RFLP method, revealed that Met326Ile polymorphism is not associated with SMA type I disease: ORMet/Met = 0.398 with a p = 0.072 meanwhile ORMet = 0.495, p = 0.063. However, the Cochrane – Armitage test indicated that there is a statistically association trend between the analyzed polymorphism and SMA type I pathology: ORMet = 0.438, p = 0.032. We concluded that additional researches with an increased subjects number and replicates studies in other populations will clarify the investigated relationship and it may contribute to the SMA molecular mechanism understanding.

Keywords: spinal muscular atrophy, PIK3R1 gene, polymorphism

 

 

RARE ASSOCIATION BETWEEN TWO GENETIC CONDITIONS: TURNER SYNDROME AND BETA THALASSEMIA MINOR

Dorina STOICANESCU*, Mariana CEVEI**, Valerica BELENGEANU*, Monica STOIAN*,
Alina BELENGEANU***

*University of Medicine and Pharmacy "Victor Babes", Department of Medical Genetics, Timisoara, Romania
**University of Oradea, Faculty of Medicine and Pharmacy, Department of Medicine, Oradea, Romania
*** University of Medicine and Pharmacy "Victor Babes", Department of Cell and Molecular Biology, Timisoara, Romania
Corresponding author: Dorina Stoicanescu, Medical Genetics Department, University of Medicine and Pharmacy “Victor Babes”, 2 E. Murgu
Square, 300041 Timisoara, Romania, tel.: 0040256204476, e-mail: dstoicanescu@yahoo.com

Abstract: Rare disorders are defined as diseases, including those of genetic origin, which are life-threatening or chronically debilitating, which are of such low prevalence that special combined efforts are needed to address them. We present a case with a rare association between two genetic conditions: Turner phenotype and beta thalassemia minor. Turner syndrome is a chromosomal disorder that is characterized by the absence of all or part of a second sex chromosome in some or all cells. This condition occurs in 1 in 2,500 to 3,000 girls. The physical features include webbing of the neck, short stature, delayed growth of the skeleton, broad chest, cardivascular abnormalities and gonadal dysgenesis. Women with this disorder are usually infertile due to ovarian failure. The clinical diagnosis was confirmed by the cytogenetic and by FISH analysis, which revealed the presence of only one X chromosome. Treatment may include human growth hormone and estrogen replacement therapy. On the other hand, thalassemias are genetic conditions that result from imbalance in the normal coordinated synthesis of the globin subunits that make up the hemoglobin tetramer, leading to decreased and defective production of hemoglobin. Beta thalassemia syndromes are hereditary disorders characterized by a genetic deficiency in the synthesis of beta-globin chains. β thalassemia is inherited in an autosomal recessive manner. Thalassemia minor usually presents as an asymptomatic mild microcytic anemia, but our case also had splenomegaly and required splenectomy.

Keywords: Turner syndrome, β thalassemia minor, rare diseases

 

 

RESEARCHES CONCERNING THE STRUCTURE AND ACTIVITY OF EDAPHIC MICROFLORA COMMUNITY WHEN GLYPHOSATE IS ADDED

Renata ŞUMĂLAN*, Ersilia ALEXA*, Monica NEGREA* Cristina DEHELEAN**

* BanatUniversity of Agricultural Sciences and Veterinary Medicine Timisoara, Faculty of Horticulture and Forestry, Timișoara, Romania
** University of Medicine and Pharmacy Timişoara, Faculty of Pharmacy, Timișoara, Romania
Corresponding author: Renata ŞumĂlan, Banat University of Agricultural Sciences and Veterinary Medicine Timisoara, Faculty of Horticulture and Forestry, Department of Soil Microbiology, 119 Calea Aradului, 300645 Timișoara, Romania, tel.: 0040256277082, fax: 0040256424232, e-mail: srenata_maria@yahoo.com

Abstract. The study was realized on two soil types: Chernozem and typical Gleysol. The results highlighted the positive effect on the microflora in the addition of glyphosate case, which is an alternative source of C, N or P.  Daily respiration rate registered highest value in the 4th day of the treatment. Quantitative and qualitative estimation of microflora revealed abundant development of the zymogene fraction exponent, respectively of bacterial species belonging to the Pseudomonas and Arthrobacter species.

Keywords: cultivable heterotrophic bacteria, actinomycetes, micromycetes, daily soil respiration rate

 

 

MOLECULAR MARKERS FOR GENETIC STABILITY OF INTERGENERIC HYBRIDS
Fragaria x Potentilla DERIVED FROM TISSUE CULTURE

Anca Nicoleta SUTAN*, Aurel POPESCU*, Raluca GHEORGHE**,
Carmen Florentina POPESCU**, Valentina ISAC***

* University of Pitesti, Faculty of Sciences, Department of Biology and Horticulture, Pitesti, Romania
** National Research and Development Institute for Biotechnology in Horticulture, Laboratory of Molecular Genetics, Stefanesti, Romania
*** Research Institute for Fruit Growing, Pitesti, Romania
Corresponding author: Anca Nicoleta Sutan, University of Pitesti, Faculty of Sciences, Department of Biology and Horticulture, 1 Targu din Vale, 110040 Pitesti, Romania, tel.: 0040348453100, fax: 0040348453123, e-mail: ancasutan@yahoo.com

Abstract. The effect of growth regulators, explant source and culture age on genetic stability of plants obtained from tissue culture propagation of ornamental strawberry “Serenata” were examined. Genomic DNAs of in vitro-derived shoots and control plant were extracted and compared by RAPD-PCR analyses. Ten primers (from 48 previously tested) were selected and used in RAPD analysis to prove the clonal fidelity (i.e. genetic stability) of the tissue culture-derived ornamental strawberry plants. The lack of polymorphisms in micropropagated plants screened through molecular markers was used to suggest genetic fidelity. Identicaly banding patterns of the RAPD profiles obtaining from vitroplants, regenerated via organogenesis or meristems culture, suggested that in the ornamental strawberry, variety “Serenata”, neither explant source, nor callus age or limited number of subcultures, in basal media supplemented with low concentration of growth regulators, were associated with occurence of somaclonal variation.

Keywords: tissue culture, intergeneric hybrids, RAPDs, clonal fidelity

 

 

THE EFFECTS OF CULTURE MEDIUM COMPOSITION ON IN VITRO ROOTING OF TWO INTERGENERIC HYBRIDS Fragaria x Potentilla

Anca Nicoleta SUTAN*, Aurel POPESCU*, Valentina ISAC**

* University of Pitesti, Faculty of Sciences, Department of Biology and Horticulture, Pitesti, Romania
** Research Institute for Fruit Growing, Pitesti, Romania
Corresponding author: Anca Nicoleta Sutan, University of Pitesti, Faculty of Sciences, Department of Biology and Horticulture, 1 Targu din Vale, 110040 Pitesti, Romania, tel.: 0040348453100, fax: 0040348453123, e-mail: ancasutan@yahoo.com

Abstract. As an important stage in micropropagating ornamental strawberry, in vitro rooting of microshoots on media containing different concentrations of auxins was investigated in two intergeneric hybrids Fragaria x Potentilla, respectively “Pink Panda” and “Serenata”. IBA at either 0.25 or 0.5 mg/l, and IAA at 0.5 mg/l concentration, were added to solidified Murashige and Skoog (1962) basal medium containing half strenght macroelements and half Lee-Fossard microelements. In all treatments, 0.1 mg/l of GA3 was also added to the basal medium. IBA was found to be the most effective auxin in promoting rhizogenesis, with the concentration 0.25 mg/l giving the highest rooting rates for both varieties, respectively 100% for “Pink Panda”, and 80% for “Serenata”.

Keywords: in vitro culture, root induction, auxins, cytokinins, intergeneric hybrids Fragaria x Potentilla.

 

 

STUDYING THE EFFECT OF ELEVATION AND EDAPHIC VARIABLES ON VEGETATION COMPOSITION IN KHEZRABAD RANGELANDS USING PRINCIPAL COMPONENT ANALYSIS (PCA)

Ruhollah TAGHIZADEH MEHRJARDI *, Ali AKBARZADEH *, Mehdi BINIYAZ **, Shahla MAHMOODI*, Mohammad Ali ZARE CHAHOUKI **

* Department of Soil Science, Faculty of Water & Soil Engineering, University College of Agriculture & Natural Resources, University of Tehran, Karaj, 31587-77871, Iran
**Department of Rehabilitation of Arid and Mountainous Regions, University College of Agriculture & Natural Resources, University of Tehran, Karaj, 31585-4314, Iran
Corresponding author: Ali Akbarzadeh, Department of Soil Science, Faculty of Water & Soil Engineering, University College of Agriculture & Natural Resources, University of Tehran, Karaj, 31587-77871, Iran, tel.: +98 9113305488, fax: +98 2612231878, e-mail: aliakbarzadeh1236@yahoo.com

Abstract. A major scientific challenge in plant ecology is to identify and quantify the strength of environmental factors that are responsible for the distribution and abundance of plant species within and among ecosystems. Hence, this study is focused on relation between plant communities and environmental variables in Khezrabad region of Iran. Based on field surveys, eight vegetation types including Artemisia sieberi-Acantholimon erinaceum, Artemisia sieberi-Hertia angustifolia, Artemisia sieberi-Launea acanthodes, Artemisia sieberi-Salsola tomentosa, Artemisia sieberi-Zygophyllum atriplicoides, Artemisia aucheri-Astrgalus albispinus, Artemisia sieberi-Fortuynia bungei, Haloxylon aphyllum were identified. With respecting to the present variance between vegetation and environmental factors, four samples were established in each vegetation type in 0–30 cm depth. The studied soil variables affecting plant communities were texture, EC, pH, Na+, k+, Cl-, Ca2+, Mg2+, SP, O.M, CaCO3, HCO3- and CEC. Among the topographic conditions, elevation was recorded in sampling regions as well. Data matrix of environmental factors and vegetation type was made using the windows (ver. 4.17) of PC-ORD. Results according to PCA showed that in the study area, among different environmental factors, the distribution of vegetation types was most strongly correlated with some agents such as soil texture, salinity and sodicity. In fact, soil texture controls distribution of plant species by affecting moisture availability, ventilation and distribution of plant roots. Beside, soil salinity and sodicity because of habitat condition, plant ecological needs and tolerance range can have negative affect on plant diversity. In addition, results indicated that increasing of elevation had negative effect on plant distribution. However, soil characteristics have more influence on vegetation separation than to the elevation in this study.

Keywords: environmental factors, Khezrabad rangelands, PCA, vegetation types.

 

 

P53 AND DCC POLYMORPHISMS AND THE RISK FOR COLORECTAL CANCER IN ROMANIAN PATIENTS – A PRELIMINARY STUDY

Mihai TOMA*, Monica STAVARACHI*, DĂnuţ CIMPONERIU*, Pompilia APOSTOL*, Mihai COJOCARU*, Laurenţiu BELUŞICĂ**, Nicolae PANDURU***, Irina RADU*, Lucian GAVRILĂ*

* Human Genetics and Molecular Diagnostic Laboratory, Institute of Genetics, University of Bucharest, Romania.
** Surgical Departament, CantacuzinoHospital, Bucharest, Romania.
*** N Paulescu Institute, Bucharest, Romania.
Corresponding author: Mihai Toma, Human Genetics and Molecular Diagnostic Laboratory, Institute of Genetics, University of Bucharest, 1-3 Intrarea Portocalelor Str., District 6, 060101 Bucharest, Romania, tel.: 0040213181576, fax: 0040213181565, e-mail: iahim_t@yahoo.com

Abstract. Inactivation of tumor suppressor genes p53 and DCC has been frequently observed in colorectal cancer. The aim of this case-control study was to test possible association between polymorphisms g.32008376A>G (rs714)of DCC geneand g.7175464A>G (rs1625895) of p53 gene and colorectal cancer risk in Romanian patients. We investigate these two polymorphisms by PCR-RFLP in individuals with colorectal cancer (n=120, M:W=74:46) and healthy persons (n=60, M:W=32:28). We observed that GG genotype of both genes confer protection for CRC (ORDCC 0.34, 95%CI 0.18-0.66, ORp53 0.28, 95%CI 0.14-0.55). The presence of DCC AA (OR 2.97, 95%CI 0.97-9.08) and p53 GA (OR 3.86, 95%CI 1.89-7.87) genotypes are associated with an increased risk for CRC. The alleles A of both markers are associated with the risk for disease (OR 2.87, 95%CI 1.49-5.50, respectively 3.54, 95%CI 1.81-6.91). We also observed that coinheritance of DCC GG genotype and p53 GG (OR 0.36) or p53 GA (OR 0.23) confer protection for CRC. These apparent discordant results obtained for the p53 gene may be the result of interaction with other markers or a selection bias. Our findings indicate that the p53 and DCC polymorphisms are associated with a risk of CRC in Romanian patients.

Keywords: colorectal cancer, p53 gene, DCC gene, genetic polymorphism, PCR-RFLP.

 

 

DATA ABOUT AN ANTHROPOMETRICAL COMPARATIVE STUDY IN TWO FEMININE POPULATIONS OF BIHOR COUNTY (ORADEA AND BORUMLACA)

Ioana Mihaela TOMULESCU*

*University of Oradea, Faculty of Sciences, Department of Biology,Oradea, Romania
Corresponding author: Ioana Mihaela Tomulescu, University of Oradea, Faculty of Sciences, Department of Biology,1 Universitatii, 410087 Oradea, Romania, tel.: 0040259408161, fax: 0040259408430, e-mail: imtomulescu@yahoo.com

Abstract. This paper is about the differences of fingers lenghts in two feminine populations of two different localities. These localities are: Oradea and Borumlaca, in Bihor county (Romania). Oradea is a locality with over two hundred thousands of inhabitants, which means the variability of some phenotipical features must be a large one. Borumlaca is a smaller locality (has under five thousands inhabitants), which means the variability of some phenotipical features is lower than in Oradea. This fact is showed in our study. We investigated 100 women from each locality. It were measured the lenghts of the 2nd, 3rd and 4th digits. The results are important: the digit lenghts are not very different in the two studied populations.

Keywords: fingers lenght, feminine populations, Oradea, Borumlaca.

 

 

 

 


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  • The traditions of higher education in Oradea, metaphorically speaking, go back to time immemorial. This year (2008) there are 228 years since the inauguration of higher education in Oradea and 44 years of continuous higher education in Oradea.
    At the end of the 18th century, "a higher institution for philosophic teaching" is founded in Oradea in 1780, which was to become in 1788 the Faculty of Law, the oldest faculty not only within the Romanian borders but also in a vast region of Eastern Europe.
    In May 1990 a decree of the Romanian Government established the Technical University of Oradea, later called the University of Oradea, and based on impressive traditions of academic life in the town. It was an act of scientific and cultural restoration long expected in the life of the Romanian society, a major gain of the people's Revolution of December 1989, one of the greatest Romanian achievements in Crisana after the Great Union on 1 December 1918. This is how the dream of several generations of scholars came true, clearly expressed by a historian of Oradea: "As regarding the future, the desire of all well-meant Romanians is to establish in Oradea a complete university, the lights of which will shine across the entire western border of Romania".
    Today, the University of Oradea is an integrated institution of higher education of this kind, comprising 18 faculties.
    The mission of the University of Oradea is to train and educate on a large scale both the students and also the high education graduates, as well as to approache certain domains of science and technology at high level.
    The structure of the University contains academic education, postgraduate education and scientific research.
    The University of Oradea expanded itself by developing new faculties and research teams, as well as by developing certain specializations inside the existing faculties.
    Education will always be the best long-term investment, as nothing is as precious as the knowledge gained during academic instruction.